LOCUS       pLV-[hsa-miR-548j]        9166 bp    DNA     circular     27-JUN-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7087..7947
                     /label=Amp(R)
     promoter        8148..8688
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5692..5926
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6501..6956)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5998..6128
                     /label=SV40 pA
     misc_feature    6129..6342
                     /label=SV40 ori
     misc_feature    5013..5609
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3631
                     /label=intron
     CDS             3678..5003
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3421
                     /label=hsa-mir-548j precursor
BASE COUNT     2174 a      2388 c      2481 g      2123 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatcct gagtggccac 
     3121 accaagatcg cctgggtctg tccaacttta ggtctacact cctgacccct gcactccacg 
     3181 acgattgcat tcctgagcct ggcccaggtg ggcagccagt gaatagttag ctggtgcaaa 
     3241 agtaattgcg gtctttggta ttactttcag tggcaaaaac tgcattactt ttgcaccagc 
     3301 ctactagaac gctgagttca gtgccttaag cggtgtttca cagagaggga gcaaggggca 
     3361 gcgcttgtta aaatgcaaat tccctcagct ccacgttaaa atgcaaattc cctcagctcc 
     3421 agctagctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat 
     3481 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta 
     3541 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac 
     3601 agtggttcaa agtttttttc ttccatttca ggtgtcgtga aaactacccc tctagagtcg 
     3661 agctaccggt cgccaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg 
     3721 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg 
     3781 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca 
     3841 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca 
     3901 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg 
     3961 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg 
     4021 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc 
     4081 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga 
     4141 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg 
     4201 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc 
     4261 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca 
     4321 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt 
     4381 acaaggaccc accggtcgcc accatgaccg agtacaagcc cacggtgcgc ctcgccaccc 
     4441 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca 
     4501 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct 
     4561 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg 
     4621 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc 
     4681 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc 
     4741 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg 
     4801 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc 
     4861 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc 
     4921 ggctcggctt caccgtcacc gccgacgtcg agtgcccgaa ggaccgcgcg acctggtgca 
     4981 tgacccgcaa gcccggtgcc tgagcggccg cgtcgacaat caacctctgg attacaaaat 
     5041 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc 
     5101 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt 
     5161 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg 
     5221 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg 
     5281 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc 
     5341 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt 
     5401 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct 
     5461 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg 
     5521 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg 
     5581 gatctccctt tgggccgcct ccccgcctgg aattcgagct cggtaccttt aagaccaatg 
     5641 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg 
     5701 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta 
     5761 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa 
     5821 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac 
     5881 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt 
     5941 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac 
     6001 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 
     6061 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 
     6121 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca 
     6181 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 
     6241 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga 
     6301 cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca 
     6361 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc 
     6421 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg 
     6481 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt 
     6541 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt 
     6601 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct 
     6661 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg 
     6721 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga 
     6781 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc 
     6841 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga 
     6901 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt 
     6961 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca 
     7021 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg 
     7081 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc 
     7141 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg 
     7201 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt 
     7261 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta 
     7321 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat 
     7381 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga 
     7441 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca 
     7501 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact 
     7561 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc 
     7621 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact 
     7681 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt 
     7741 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt 
     7801 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt 
     7861 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata 
     7921 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag 
     7981 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat 
     8041 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa 
     8101 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca 
     8161 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt 
     8221 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg 
     8281 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc 
     8341 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga 
     8401 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc 
     8461 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc 
     8521 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca 
     8581 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg 
     8641 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta 
     8701 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct 
     8761 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag 
     8821 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa 
     8881 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc 
     8941 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg 
     9001 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg 
     9061 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc 
     9121 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgc 
//
